Calcium Saccharin USP NF FCC Food Grade Manufacturers Exporters

Saccharin Calcium USP Grade Specifications:
C14H8CaN2O6S2-3½ H2O - 467.48
C14H8CaN2O6S2 - 404.44
1,2-Benzisothiazol-3(2H)-one, 1,1-dioxide, calcium salt, hydrate (2:7);
1,2-Benzisothiazolin-3-one 1,1-dioxide calcium salt hydrate (2:7) CAS 6381-91-5
Anhydrous CAS 6485-34-3

DEFINITION
Saccharin Calcium contains NLT 99.0% and NMT 101.0% of C14H8CaN2O6S2 , calculated on the anhydrous basis.

IDENTIFICATION
A. Infrared Absorption
Sample: Dry at 105C to constant weight.
B. Procedure
Sample solution: 100 mg/mL
Analysis: To the Sample solution add 2 drops of methyl red, and neutralize with 6 N ammonium hydroxide. Add 3 N hydrochloric acid, dropwise, until the solution is acid to the indicator. Add ammonium oxalat.
Acceptance criteria: A white precipitate is formed when the ammonium oxalate is added. This precipitate is insoluble in 6 N acetic acid but dissolves in hydrochloric acid.
C. Calcium salts moistened with hydrochloric acid impart a transient yellowish-red color to a nonluminous flame.

ASSAY
Procedure
Sample solution: 3 mg/mL of Saccharin Calcium in glacial acetic acid. [Note: Slight heating may be needed to dissolve the sample.]
Analysis: Titrate Sample solution with 0.1 N perchloric acid, determining the endpoint potentiometrically. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 20.22 mg of C14H8CaN2O6S2.
Acceptance criteria: 99.0%–101.0% on the anhydrous basis.

Procedure 1: Limit of Toluenesulfonamides: To pass the test.
Impurity:
o-Toluenesulfonamide: 10 ppm maximum.
p-Toluenesulfonamide: 10 ppm maximum.
Procedure 2: Limit of Benzoate and Salicylate:
Sample solution: 50 mg/mL
Analysis: To 10 mL of the Sample solution add 5 drops of 6 N acetic acid and 3 drops of ferric chloride.
Acceptance criteria: No precipitate or violet color appears.
Water Determination: NMT 15.0%
Readily Carbonizable Substances:
Sample solution: 40 mg/mL in sulfuric acid (94.5%–95.5% (w/w) of H2SO4), 48C – 50C for 10 min
Acceptance criteria: The Sample solution has no more color than Matching Fluid A, when viewed against a white background.
Clarity of Solution: To pass the test.
Color of Solution: To pass the test.
Packaging and Storage: Preserve in well-closed containers. Store at room temperature.

Calcium Saccharin FCC Food Grade Specifications
1,2-Benzisothiazolin-3-one 1,1-Dioxide Calcium Salt
C14H8CaN2O6S2-31-2H2O Formula weight 467.48
INS: 954 CAS anhydrous 6485-34-3

DESCRIPTION
Calcium Saccharin occurs as white crystals or as a white, crystalline powder. One gram is soluble in 1.5 mL of water.
Function: Nonnutritive sweetener.

REQUIREMENTS
Identification:
A. Dissolve about 100 mg of sample in 5 mL of a 1:20 solution of sodium hydroxide, evaporate to dryness, and gently fuse the residue over a small flame until ammonia no longer evolves. After the residue has cooled, dissolve it in 20 mL of water, neutralize the solution with 2.7 N hydrochloric acid, and filter. Add 1 drop of ferric chloride to the filtrate. A violet color appears.
B. Mix 20 mg of sample with 40 mg of resorcinol, cautiously add 10 drops of sulfuric acid, and heat the mixture in a liquid bath at 200C for 3 min. After cooling, add 10 mL of water and an excess of 1 N sodium hydroxide. A fluorescent green liquid results.
C. A1:10 aqueous solution gives positive tests for Calcium.
D. Add 1 mL of hydrochloric acid to 10 mL of a 1:10 aqueous solution. A crystalline precipitate of saccharin forms. Wash the precipitate well with cold water, and dry at 105C for 2 h. The saccharin thus obtained melts between 226C and 230C.
Assay: Not less than 98.0% and not more than 101.0% of C14H8CaN2O6S2, calculated on the anhydrous basis.
Benzoate and Salicylate: Passes test.
Lead: Not more than 2 mg/kg.
Readily Carbonizable Substances: Passes test.
Selenium: Not more than 0.003%.
Toluenesulfonamides: Not more than 0.0025%.
Water: Not more than 15.0%.

CAS Number 6381-91-5 for Hydrate and 6485-34-3 for Anhydrous, Calcium Saccharin manufacturers suppliers exporters at:

manufacturer supplier exporter
Aadhunik Industries
Ankleshwar Gujarat & Mumbai, India
TEL: (OFFICE) 91-22-65290104, 91-22-65290105.